Pine Mushroom Season

Last year Pine mushrooms made me make a delightful pasta dish. This year it wasn't much different and the delights went with some other mushroom

and this

and this from the balcony garden

and turned into this.

YUM! I love Pine Mushroom season at the Victoria Markets (even if they are a little expensive.)

Crazy Cukes

Before we hit crazy cuke land let's remember what a normal one looks like. This is a usual bush cucumber that I grow.

And here are three of a normal variety that I also grow.

And now there is one crazy cuke - my lovely piggy tail cucumber. Here he is growing, just to prove there was no tampering. His uniqueness was just mother nature having a giggle.

And then, finally, I picked him.

He was enjoyed in a salad both last night and today. Inside him was a sweet and slightly oversized-seed-filled centre, no different to the straight cukes. I love mother nature's little oddities. Any random growings in your gardens this year?

Harvest Monday

I am so so so close to getting the first full draft of the thesis done, but yet so far from the end goal. In the meantime here is what I have been harvesting of late ...

There have been cucumbers, plenty of cucumbers, enough that I have to eat them every day to keep up, but here is just one. I am going to post soon about the world's funniest cucumber which is shaped like a piggies tail but for now it is normal cucumbers.

The seeds were a little big, maybe I left it on there too long, but it is hard to keep up with them. Tasty in a salad.

That was my lunch, these little red lovelies went in something else,

my breakfast!

I have been going through a phase of eating fruit salad in the morning, usually blueberries and rockmelon with whatever else is affordable at the markets. It was lovely to be able to supplement it with my own home grown strawberries.

In the garden harvest there were eggplants and beans, both of which made it into curries.

I am planning on trying salt and pepper eggplant with some of the rest. I have been harvesting chillis to give to friends, basil leaves for lasagnes and silverbeet as needed. Even in this strange weather it seems to be putting out some good food. With more eggplants, the crazy corn possibly beginning to come good and the figs turning colours it might be an interesting harvest next week. For more harvests from around the world pop on over to Daphne's Dandelions.

Breeding for the Blue Tomato

Our darkest F2 fruit

Blue tomatoes, not really - the fruit are really more of an indigo color; the pigmentation more like an eggplant.  So what's the fuss about and what's the genetic basis for the accumulation of anthocyanin pigment in tomato fruit?  Here's what we've learned from the literature and from our experience in breeding for the trait.

In the last several years there’s been significant interest in breeding tomatoes with improved nutritional quality.  One approach has been to increase fruit concentration of anthocyanin and related phenolic compounds that also have anti-oxidant activity.  Professor Jim Meyers, and various graduate students working with him at Oregon State University (including Jones, Mes, Boches and Dalotto), have used TGRC lines to introgress, into cultivated tomato, two genes from wild relatives that independently regulate anthocyanin synthesis in tomato fruit.  This group has published most of what is known about the genetic basis for the trait (see Jones, 2003 link).  We are among a group of several tomato breeders working on incorporating these genes into various tomato breeding lines.  The Aft/atv parent we used in crossing was a single plant selected in our breeding nursery from OSU Blue for intense anthocyanin accumulation in the fruit and acceptable horticultural type.  Taste of this OSU Blue "donor plant" was not very good.

Aft fruit cross section - pigmentation
accumulation limited to surface
layers of cells  (P. Boches photo)

The Aft (anthocyanin fruit) gene, from S. chilense, is a regulatory gene (An2 transcription factor) that is induced by UV light and that triggers up-regulation of anthocyanin biosynthesis in tomato fruit, and to a lesser extent in vegetative tissue.  In Aft plants anthocyanin accumulates in and just below the fruit epidermis, where light penetrates the first few layer of cells.  It is believed that such accumulation is a stress and protection response to UV light (think melanin accumulation in human skin cells in response to UV light – tanning).  Typically those parts of the fruit exposed to light will accumulate anthocyanin and turn black/purple, while those parts of the fruit not exposed to light will have the normal background fruit color.  Anthocyanin accumulation begins very early during the green fruit stage and masks normal fruit color in mature fruit.  Anthocyanin appears to accumulate preferentially in the green stripes of gs and Fs fruit.

 Todd Dalotto photo

The atv (atroviolaceum) gene, from S. cheesmaniae, induces anthocyanin accumulation in vegetative tissue and, in combination with Aft, to anthocyanin accumulation in tomato fruit.  Plants homozygous for atv accumulate anthocyanin in leaves, stems and petioles, especially in conditions with high light and cool temperatures. 

Co-expression of Aft and atv have a synergistic effect on fruit anthocyanin production (see Povero 2012 link).  For this reason a breeding program for anthocyanin accumulation in tomato fruit needs to select for both Aft and atv.  Here’s what we have learned from evaluating several hundred plants from six F2 populations segregating for both Aft and tav:

Under cool high light conditions seedlings homozygous for atv will begin accumulating anthocyanin in the cotyledons and the epicotyl.  This accumulation will become more obvious as the seedlings get larger.  Maintaining cool temperatures and high light is critical for successful selection of atv/atv seedlings at this stage.

F2 seedlings segregating for
pigmentation in cotyledons

This dark purple PL seedling did not produce

fruit w/ any anthocyanin pigmentation

Once the seedlings establish in the field and the temperatures increase, the anthocyanin accumulation in leaves/stems fades quickly.  Later in the season when the temperature begins to drop, anthocyanin accumulation in vegetative tissue (especially growing points) starts anew.

Differences in pigmentation at transplanting within a F2 family,
each selected weeks earlier for seedling "purpling"

Some of seedlings with the darkest “purple” foliage had no anthocyanin accumulation in the fruit (atv positive/Aft negative).  In fact there was no significant relationship between seedling score for anthocyanin and anthocyanin accumulation in fruit.  This was a surprise, and suggests not being too rigorous in screening seedlings.  In 2010 we selected < 5% of the F2 seedlings for transplanting – based on the anthocyanin phenotype in seedlings.  This is probably more severe than needed.  Among seedlings showing some anthocyanin acculation, there is a considerable variation in degree of purpling (see photo above).  This may be an interaction between atv and Aft or other unknown genes.

Early fruit pigmentation 

Pigmentation is local and only where fruit is exposed
to direct sunlight

Anthocyanin shading on the sunny side of this F2 fruit

4    Anthocyanin accumulation in fruit (primarily due to Aft) begins very early in fruit development – but only in young fruit exposed to light.  Because early fruit is often deep in the canopy, we do some early pruning to improve light exposure, enabling the Aft phenotype.  Late in the season, with better fruit exposure to sunlight and cooler temperatures, we saw stronger expression of the Aft phenotype.

5    Among F2 segregants showing some anthocyanin accumulation in fruit, there was very significant variation in early season intensity of the pigment, and how sensitive such accumulation was to light.  Part of this may be due to whether Aft was homozygous vs heterozygous (i.e. partial dominance – suggested by Jones), but it appears there may also be interactions with other unknown genes.  We should learn more from the F3 generation.

Our best tasting F2 - a bicolor orange w/ indigo highlights

6    In the F2 generation there didn’t appear to be any relationship between anthocyanin accumulation in the fruit and flavor/taste.  Taste of fruit from the best F2 plants with a strong Aft fruit phenotype were 7.5-8.0 on a scale of 10.  There’s still some work to do here.  This year we will have F3 progeny from the best Aft lines and backcrosses of these to our best tasting non-Aft breeding lines.

Aft/gs

Indigo striped cherry

      As described in our “Genetic control of fruit stripes” blog, we saw a preferential accumulation of anthocyanin in green stripes in fruit of F2 plants co-segregating for gs and Fs.  This should yield some pretty interesting and novel looking F3 fruit in Aft/gs  and atv/gs plants with various fruit color/shape combinations.  Purple Dragon is Dean Slater's name for a F3 line combining Aft/gs.  Black and Brown Boar was the striped parent in the F1, which I think is evident here.

Late season w/ plenty of light exposure to fruit and cooler temperature

High pigment mutants hp1 and hp2 dg are lesions in regulatory genes (transcription factors) that regulate light-signaling in tomatoes.  Homozygous hp plants show several novel developmental and metabolic phenotypes, including increased fruit pigmentation and accumulation of various flavonoid phytonutrients.  A combination of hp1 or hp2 dg with Aft and atv show >20x enhanced anthocyanin accumulation in tomato fruit, see photo here.  The hp  phenotype generally also results in negative effects on plant vigor and fruit yield.  The combination of hp and Aft/atv is subject to a U.S. patent application (patent application link).

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Here's a 2012/13 update.  With a few exceptions it appears that to get exceptional flavor in an Aft/atv line it will require two generations of crossing (a cross and a backcross/modifed backcross) of P20 to a high flavor parent.  We have several nice MBC1 lines now in the breeding program  One exception is the Indigo Tiger cherry shown above and now in the F5.  In 2012 I planted the new OSU release Indigo Rose (OSU press release).  Good anthocyanin expression but flavor was just fair, not good enough for me to want to plant again.  I've also tried several of Tom Wagner's Aft lines and found a few interesting plants in these widely segregating populations.  One of the best was a green when ripe (GWR) line with anthocyanin stripes, from a line he called Muddy Waters.  We used this in several crosses in 2012, and some interesting F2s from these in 2013.

Selection from Muddy Waters

MW x GWR breeding line - F2

We had a chance to visit Brad Gates (Wild Boar Farms) in 2012 and he had several Aft/atv lines, including a couple of ours.  Here's a photo from his nursery of an Aft line tracing to a breeding line of ours we call Freckled Strawberry.  Note the indigo freckles and shoulder on this unripe fruit.  The bottom photo is from a similar Aft Freckled Stawberry line from our 2013 Pennsylvania nursery - top fruit from within the canopy (no light, no anthocyanin) and bottom fruit exposed to light on one side with anthocyanin present.

Aft Freckled Strawberry

We've found another interesting manifestation of Aft/atv in a segregating breeding line tracing to our cross P20 x Beauty King.  Note the "tiger stripes" pattern.  Although we are yet unable to stabilize the phenotype, anthocyanin accumulates in lateral stripes on developing and ripe fruit (coexpression w/ Fs gene).  The Fs phenotype appears to be highly influenced by environment, complicating the breeding.  This photo is also from our 2013 Pennsylvania nursery - nickname for now is Bengal Tiger
.

Bengal Tiger